Skip to main content
. 2011 Dec 13;287(5):3598–3608. doi: 10.1074/jbc.M111.281030

FIGURE 2.

FIGURE 2.

Expression and purification of the components of VcSiaPQM. A, Coomassie-stained SDS-polyacrylamide gel of the purification of the VcSiaQM complex (His-tagged on the N terminus of VcSiaQ) using IMAC. Lane 1, molecular weight ladder; lane 2, total membrane vesicles; lane 3, column flow-through; lane 4, 40 mm imidazole washing step; lanes 5–7, 500 mm imidazole elution steps. B, Coomassie-stained SDS-polyacrylamide gel of N-terminally tagged VcSiaP purified in one IMAC step. Lane 1, molecular mass marker with the sizes of the proteins indicated in kDa; lane 2, IMAC-purified VcSiaP.