Table 4.
Differences among ligation methods used for the ligation comparison
| Ligation temperature (°C) | |||
|---|---|---|---|
| Chemicals | Cycle program | Tested range | Published |
| DTM [17] | DTM | 46-65* | 50 |
| PPLMD II [10] | PPLMD II | 59.3-75* | 72 |
| LPA [12] | LPA | H:51.7-65*; L:54 | H:60 / L:54 |
| PPLMD I [8] | PPLMD I | 53-68* | 65 |
| PPLMD I | DTM | 50; 55.5; 59.3; 65 | 50 |
| PPLMD II | 61; 65; 67.8; 72 | 72 | |
| LPA | H:60 / L:54 | H:60 / L:54 | |
| H:71 / L65 | |||
| H:65 / L:59 | |||
| H65 / L:65 | |||
| SNPlex [15] | Ramp 1: 60-51 | 3% Ramp: 60-51 | |
| Ramp 2: 74-65 | |||
| Ramp 3: 70-61 | |||
* Range with eight different temperatures. H: hybridisation, L: ligation