Table 5.
Results of the ligation comparison using different chemicals with different cycle conditions
| ΔCt (SD) | |||||||
|---|---|---|---|---|---|---|---|
| Target | GM mixes | Target % | PPLMD I - 65°C | DTM - 63.6°C | LPA - 62.4°C | PPLMD II - 62.5°C | p-value ANOVA |
| hmg | 1 | 100 | 14.3 (0.4)a | 8.1 (0.7)b | 6.9 (0.4) bc | 3.8 (3.8) c | 5.7E-05 |
| 2 | 100 | 13.8 (0.9) a | 8.1 (0.3) b | 6.4 (0.4) bc | 3.3 (3.5) c | 2.6E-05 | |
| 3 | 5 | 7.8 (1.6) | ND | ND | ND | - | |
| bar | 1 | 0.1 | ND | - | |||
| 2 | 2.5 | 3.9 (0.5) | 4.5 (2.3) | 1.0 (0.4) | ND | > 0.01 | |
| 3 | 2.5 | 3.5 (1.2) ab | 4.8 (2.3) a | 0.8 (0.6) b | 0.6 (0.5) b | 2.3E-03 | |
| cry1Ab | 1 | 0.1 | ND | - | |||
| 2 | 2.5 | 7.5 (0.0) a | 8.2 (0.1) a | 3.2 (1.0) b | 0.5 (0.2) c | 1.1E-10 | |
| 3 | 2.5 | 6.9 (0.5) b | 8.3 (0.7) a | 3.4 (0.6) d | 0.6 (0.4) d | 6.3E-10 | |
| TC 1507 | 1 | 5 | ND | - | |||
| 2 | 1 | ND | |||||
| 3 | 2.5 | ND | |||||
SD: standard deviation of the ΔCt values
Values marked with different superscript characters indicate groups (for the same target-mix combination) that are significantly different according to Tukeys HSD test (P < 0.05); xa is different from yb, z abis not different from either.
ND: not detected or negative ΔCt.
The temperature shows the optimal temperature from which the data were analysed.