Table 6.
Results of the ligation comparison using chemicals from PPLMD I protocol with different cycle conditions
| ΔCt (SD) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Target | GM mixes | Target % | PPLMD I 65°C | DTM 65°C | LPA H60-L 54°C |
PPLMD II 65°C |
SNPlex Ramp 70-61°C | p-value ANOVA |
| hmg | 1 | 100 | 14.3 (0.4) a | 10.0 (0.7) c | 9.0 (1.4) c | 12.0 (0.2)b | 12.6 (0.7)ab | 1.2E-06 |
| 2 | 100 | 13.8 (0.9) a | 10.5 (1.0) cd | 9.3 (0.9) d | 12.6 (0.7) ab | 12.0 (0.2) bc | 6.5E-06 | |
| 3 | 5 | 7.8 (1.6) | 5.2 (1.3) | 4.7 (2.2) | 7.5 (1.1) | 7.1 (0.2) | > 0.01 | |
| bar | 1 | 0.1 | ND | |||||
| 2 | 2.5 | 3.9 (0.5) | 2.5 (0.7) | 4.5 (3.19) | 4.0 (0.7) | 3.2 (1.0) | > 0.01 | |
| 3 | 2.5 | 3.5 (1.2) | 2.5 (1.3) | 3.4 (3.5) | 3.3 (1.3) | 3.9 (0.7) | > 0.01 | |
| cry1Ab | 1 | 0.1 | ND | |||||
| 2 | 2.5 | 7.5 (0.0) b | 5.2 (0.5) b | 4.4 (2.5) b | 11.4 (0.6) a | 7.5 (2.3) b | 1.2E-04 | |
| 3 | 2.5 | 6.9 (0.6) b | 5.7 (0.5) b | 5.4 (2.4) b | 10.9 (0.3) a | 7.5 (2.1) b | 5.7E04 | |
| TC 1507 | 1 | 5 | ND | |||||
| 2 | 1 | ND | ||||||
| 3 | 2.5 | ND | ||||||
SD: standard deviation.
Values marked with different superscript characters indicate groups (for the same target-mix combination) that are significantly different according to Tukeys HSD test (P < 0.05); xa is different from yb, zab is not different from either.
ND: not detected or negative ΔCt.
The temperature shows the optimal temperature from which the data were analysed.