Fig. 2.

Sections from the optic nerve head region of a 7-WG embryonic eye (optic nerve to the right). A: The H&E-stained section shows round and spindle-shaped cells in the innermost retina and within the developing nerve fiber layer (arrowheads). B: CD31 was only present in the developing choriocapillaris (arrow indicates the bleached RPE in all panels). C: CD39 was present in cells in the nerve fiber layer (arrowheads). Staining was also seen in the developing choriocapillaris. D: CD45⁺ cells were scattered throughout choroid and some in the vitreous cavity. E: CXCR4 was observed throughout the inner neuroblastic layer and within cells of the nerve fiber layer (arrowheads), similar to those in the H&E (A) and the cells labeled with CD39 (C). F: Ki67 was only associated with cells within the outermost portion of the neuroblastic layer of retina and within the choroid. B–F: APase reaction product.