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. 2012 Feb 2;8(2):e1002492. doi: 10.1371/journal.pgen.1002492

Figure 1. Overexpression of CIK1-CC disrupts the Cik1–Kar3 interaction and phenocopies the growth defects of cik1 and kar3 mutants.

Figure 1

A. Overexpression of CIK1-CC abolishes Cik1-Kar3 interaction. CIK1-13myc KAR3-3HA cells with a vector (V) or a PGALCIK1-CC (CC) plasmid were grown to mid-log phase in raffinose medium at 30°C. After galactose was added to the cell cultures for 3 hrs, the cells were collected for immunoprecipitation (IP) with anti-HA antibody and the precipitates were subjected to Western blotting with anti-HA and anti-myc antibodies. B. Overexpression of CIK1-CC phenocopies cik1Δ and kar3Δ mutants. Cell cultures in stationary phase with indicated genotypes were 10-fold diluted and spotted onto glucose or galactose plates with or without 100 mM HU. The plates were scanned after incubation at 25°C or 37°C for 3–4 days.