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. 2012 Feb 2;8(2):e1002507. doi: 10.1371/journal.ppat.1002507

Figure 2. Unlike the attenuated BCG vaccine, virulent M. tuberculosis is able to induce phagosomal rupture in THP-1 macrophages.

Figure 2

THP-1 cells were infected with BCG DsRed (A,C) or M. tuberculosis DsRed (B,D) at a MOI of 1 for the indicated time and then loaded with CCF-4 molecule for 2 h. After PFA fixation, cells were imaged using a fluorescence widefield microscope (Nikon Ti) with an 40X objective (A, B). Picture acquisition was achieved randomly and automatically for each condition on 49 fields in duplicates and further 450/535 nm intensity ratio measurements shown in panels C and D were obtained through analysis by a specialized algorithm using the Metamorph software. The plots are representative of 3 independent experiments.