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. 2012 Feb 2;8(2):e1002496. doi: 10.1371/journal.ppat.1002496

Figure 3. Both viral and mammalian cyclin-expressing viruses confer endothelial cell survival and persistence.

Figure 3

(A) Schematic representation of persistent infection of endothelial cells for viability determination. (B) MB114 endothelial cells were infected with recombinant viruses for 6 days, at which time non-adherent cells were collected and analyzed for viability by dual PI and annexin V staining. Flow cytometry zebra plots are representative of 3 independent experiments from gHV-cycV, gHV-cycK, gHV-cycA, gHV-cycE, gHV-cycD3 and gHV-cycD2 virus infections. (C) Bar graph of mean viability (annexin V-/PI-) from flow cytometric analysis of 3 independent experiments. Data was found to be statistically significant between gHV-cycKO compared to WT (*p = 0.001), gHV-cycV (*p = 0.004), gHV-cycK (*p = 0.001), gHV-cycE (*p = 0.001) and gHV-cycA (*p = 0.001) as determined by unpaired t-test.