Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Feb 2;8(2):e1002497. doi: 10.1371/journal.ppat.1002497

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Viejo-Borbolla et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Sensorgrams depicting the interaction between chemokines and SgG1 (left) or SgG2 (right). The indicated chemokines were injected at a 100 nM concentration. The arrow indicates the end of injection. All curves were analyzed with the BiaEvaluation software and represent the interaction of the chemokine after subtraction of the blank curve. Only 4 out of 11–12 positive interactions are shown. Abbreviations: Diff. Resp., Differential response; R.U., response units; s, seconds (B, C) Crosslinking assays showing the interaction of HSV-SgGs with [¹²⁵I]-hCXCL10 (B) and [¹²⁵I]-hCCL25 (C). Recombinant purified HSV-SgGs were incubated with iodinated chemokine and crosslinked with EGS (for [¹²⁵I]-hCCL25) or BS³ (for [¹²⁵I]-hCXCL10). The samples were resolved by SDS-PAGE, fixed and visualized by autoradiography. (D) Crosslinking assay between [¹²⁵I]-hCXCL12α and SgG2 in the presence of increasing concentrations of cold hCXCL12α. Molecular masses are indicated in kDa. SgG-chemokine complexes are indicated with arrows and crosslinked chemokine dimers are marked with asterisks. Abbreviations: CRD, CrmB-cysteine rich domain.