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. 2012 Feb 2;7(2):e30716. doi: 10.1371/journal.pone.0030716

Table 1. Sequences, efficiency and annealing temperature of primers.

Gene Primer sequences Efficiency Annealing temperature
Adiponectin senseantisense 5′-GCCTCTTCAAGAAGGACAAGGCTATG -3′ 5′-CAGTTGGTGTCATGGTAGAGAAG -3′ 1.93 69°C
AdipoR1 senseantisense 5′-ACTGGAGCTGGCCTTTATGCTGC -3′ 5′-AGAGAAGGGTGTCATCAGTACAGC -3′ 2.04 69°C
AdipoR2 senseantisense 5′-CCATAGGGCAGATAGGCTGGTTGA -3′ 5′-CAGTGCATCCTCTTCACTGCAGC -3′ 1.97 69°C
β-actin senseantisense 5′-CATGGATGATGATATCGCCGCG-3′ 5′-ACATGATCTGGGTCATCTTCTCG-3′ 1.84 69°C
HMOX1 senseantisense 5′-CCAGGCAGAGAATGCTGAGTTCAT-3′ 5′-CCGTACCAGAAGGCCAGGTCC-3′ 1.97 69°C
IL1β senseantisense 5′-ATGGCAGAAGTACCTGAGCTCGC-3′ 5′-TTAGGAAGACACAAATTGCATGGTG-3′ 1.83 68°C
IL6 senseantisense 5′-ATGAACTCCTTCTCCACAAGC-3′ 5′-CTACATTTGCCGAAGAGCCC-3′ 2.12 68°C
IL8 senseantisense 5′-ATGACTTCCAAGCTGGCCGTGG-3′ 5′-TGAATTCTCAGCCCTCTTCAAAAAC-3′ 2.02 68°C
IL10 senseantisense 5′-TTAAGGGTTACCTGGGTTGC-3′ 5′-GCCTTGCTCTTGTTTTCACA-3′ 1.94 65°C
MMP1 senseantisense 5′-ATGCACAGCTTTCCTCCACTGC-3′ 5′-CACTGGGCCACTATTTCTCCGC-3′ 2.05 69°C
MMP3 senseantisense 5′-ATCGATGCAGCCATTTCTGATAAGG-3′ 5′-TCAACAATTAAGCCAGCTGTTACT-3′ 2.06 69°C
Involucrin senseantisense 5′-CCCAGCAACACACACTGCCAGT-3′ 5′-GCTCAGGCAGTCCCTTTACAGCA-3′ 1.98 69°C
TGFβ1 senseantisense 5′-GAGCCCTGGACACCAACTAT-3′ 5′-GACCTTGCTGTACTGCGTGT-3′ 1.94 69°C
KGF senseantisense 5′-AGTTGGAATTGTGGCAATCA-3′ 5′-CCGTTGTGTGTCCATTTAGC-3′ 1.84 65°C