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. Author manuscript; available in PMC: 2012 Feb 2.
Published in final edited form as: IEEE Trans Med Imaging. 2011 Jul 14;31(1):16–32. doi: 10.1109/TMI.2011.2162099

Fig. 2.

Fig. 2

Comparison of standard transmitted visible light images and confocal images of reflected visible light to demonstrate the through-plane resolution achievable by applying the confocal technique to back-scattered light of Golgi-stained brain tissue. (a) A transmitted light image of a Golgi field within cerebral cortex is shown. Panels (b)–(e) show side-by-side comparisons of the indicated subregion of (a) obtained from transmitted (b), (c) and confocal microscopy of reflected (d), (e) visible light. The focal plane of image pairs (b), (d) is offset relative to (c), (e) by 15 μm. Confocal microscopy of reflected light enables sufficient through-plane resolution to be achieved to construct 3-D models of cellular structures, whereas out-of-plane structures in transmitted light images observable in (b), (c) would interfere with subsequent steps of the 3-D analysis.