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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Cancer Res. 2011 Dec 12;72(3):604–615. doi: 10.1158/0008-5472.CAN-11-0669

Figure 6. Effect of macrophage depletion on tumor growth and angiogenesis.

Figure 6

(A) Growth of MVT-1 derived tumors in Dox- induced or uninduced MMTV-mS100a7a15 mice treated with either clodrolip or control-liposomes. (B) Representative photograph of tumors derived from different experimental groups. (C) Quantitative analyses of F4/80+ macrophages (white columns) or CD206+ M2 macrophages (black columns) by FACS. Graphs represent the mean ± SD (Control, n = 4; clodrolip, n = 5) *, P < 0.05; **, P < 0.01. (D) Representative IHC staining of mammary tumor sections treated with clodrolip and control-liposomes with macrophage marker F4/80 antibody (upper) and with endothelial marker CD31 antibody (lower) to assess the number of macrophages infiltrating into tumors and increase angiogenesis in tumors from Dox-treated compared to untreated mice. (E) Bars represent the mean ± SD of the number of F4/80+ macrophages (left) and CD31+ blood vessels (right) as shown in D and counted in five random high power fields (HPF, 20X) per tissue section (Control, n = 4; clodrolip, n = 5) *, P < 0.05; **, P < 0.01.