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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Cell Biol. 2011 Dec 18;14(2):186–191. doi: 10.1038/ncb2404

Figure 4.

Figure 4

Wnt7a induces hypertrophy independent of IGF-receptor activity. (a) Immunoblot analyses revealed no changes in the phosphorylation of IGF receptor as well as IRS-1 in Wnt7a-HA expressing cells compared to control cells. Additionally the total amount of IGF-receptor and IRS1 were not significantly changed in Wnt7a-HA expressing cells compared to control cells. n=3. (b) Inhibition of IGF receptor activity by AG1024 did not result in changes in fibre diameter in Wnt7a-HA expressing cells suggesting that Wnt7a acts independently of IGF receptor activity. All analyses were performed in cells which were differentiated for 5 days. n=3. (c) Quantitative Real-time PCR of members of the IGF and IGF receptor family (n=3), *** p<0.0001. Error bars represent SEM. See S6 for uncropped images of blots.