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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Acta Physiol (Oxf). 2011 Oct 25;204(4):562–570. doi: 10.1111/j.1748-1716.2011.02367.x

Fig. 1. Effect of phloretin on glycerol and FFA efflux.

Fig. 1

a. Intracellular fluorescence of 3T3-L1 adipocytes treated with 0uM (black) or 500uM phloretin (grey) detected after 30-min incubation with forskolin/IBMX. Phloretin was added at the beginning of the assay. n=6 Colorimetric assays to determine glycerol (b) and FFA (c) efflux over time from forskolin/IMBX stimulated 3T3-L1 adipocytes in the presence of 0 µM (squares) or 500µM phloretin (triangles). Lines indicate linear regression through data points to calculate efflux rates. n=3