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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Acta Physiol (Oxf). 2011 Oct 25;204(4):562–570. doi: 10.1111/j.1748-1716.2011.02367.x

Fig. 2. Modification of cellular efflux.

Fig. 2

Glycerol (a) and FFA (b) efflux rates from forskolin/IMBX stimulated 3T3-L1 adipocytes in the presence of phloretin, verapamil, glyburide (n=8), or DIDS (n=3) at the indicated concentrations.

c. 3H triolein hydrolysis assay in the presence of 400µM phloretin or glyburide. n=3 cell lysates

d. Intracellular FFA concentration in control, phloretin (500µM) or DIDS (250µM) treated, forsklolin/IBMX stimulated 3T3-L1 adipocytes. n=6

e. Inhibition of 14C-oleate efflux from 3T3-L1 adipocytes by DIDS 30-minutes after initial lipolytic stimulation. Nonlinear fit of data indicates an IC50 of 102µM.