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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Acta Physiol (Oxf). 2011 Oct 25;204(4):562–570. doi: 10.1111/j.1748-1716.2011.02367.x

Fig. 4. HFP cells are a reconstituted cellular model for FFA efflux.

Fig. 4

a. Western blot of HFP (lane 1), 3T3-L1 fibroblast (lane 2), and 3T3-L1 adipocyte (lane 3) lysates probed for stable expression of hormone sensitive lipase (HSL), FATP1, and perilipin (Plin).

b. 3D reconstruction of confocal images of HFP cells following overnight lipid loading in the presence of fluorescent fatty acids (green); nuclei are shown in blue.

c. Analysis of lipid droplet diameter based on 3D reconstruction shown in B. n=5200 lipid droplets

d. Intracellular fluorescence of HFP cells incubated overnight with lipids and C1-BODIPY-C12 stimulated with forskolin/IBMX at time=0. n=4

e. Mean fluorescene of HFP cell populations loaded overnight with lipids and C1-BODIPY-C12 and stimulated for 30min with insulin, forskolin/IBMX, or nothing. n=4

f. Dose-dependent effect of DIDS on FFA efflux rate from forskolin/IBMX stimulated HFP cells and 3T3-L1 adipocytes. n=3