Table 1.
Gene | Acc. No. | Primera |
---|---|---|
CCN3/NOV | NM_030868 | for: CTGAGATGAGACCCTGCGAC |
rev: CTCTGAGCCACAGGTCCACT | ||
GAPDH | NM_017008 | for: TGTGGATCTGACATGCCGCC |
rev: AACCACCCTGTTGCTGTAGC | ||
CCN3/NOV | NM_030868 | for: CTACAGAGTGGAGCGCGTGTT |
rev: GGAAGATTCCTGTTGGTGACCC | ||
probe: AAGAGCTGTGGAATGGGCTTGTCCAC | ||
CCN2/CTGF | NM_001010 | for: AGCAGCTGGGAGAACTGTGC |
rev: GCCGAAGTCGCAGAAGAGAC | ||
probe: CGTGATCCCTGCGACCCACACAA | ||
Col type I(α) | NM_053304 | for: GAAGGCAACAGTCGATTCACC |
rev: GACTGTCTTGCCCCAAGTTCC | ||
probe: ACAGCACGCTTGTGGATGGCTGC | ||
α-SMAb | NM_031004 | for: GAGGAGCATCCGACCTTGC |
rev: ATTTTCTCCCGGTTGGCC |
a the first two primer sets were used in conventional PCR, all others primers were used for quantitative real-time PCR analysis on TaqMan.
bthe quantification of α-SMA was done with the SybrGreen dye.