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. 2011 Jul 5;6(1):11–25. doi: 10.1007/s12079-011-0141-3

Table 1.

Primers used in this study

Gene Acc. No. Primera
CCN3/NOV NM_030868 for: CTGAGATGAGACCCTGCGAC
rev: CTCTGAGCCACAGGTCCACT
GAPDH NM_017008 for: TGTGGATCTGACATGCCGCC
rev: AACCACCCTGTTGCTGTAGC
CCN3/NOV NM_030868 for: CTACAGAGTGGAGCGCGTGTT
rev: GGAAGATTCCTGTTGGTGACCC
probe: AAGAGCTGTGGAATGGGCTTGTCCAC
CCN2/CTGF NM_001010 for: AGCAGCTGGGAGAACTGTGC
rev: GCCGAAGTCGCAGAAGAGAC
probe: CGTGATCCCTGCGACCCACACAA
Col type I(α) NM_053304 for: GAAGGCAACAGTCGATTCACC
rev: GACTGTCTTGCCCCAAGTTCC
probe: ACAGCACGCTTGTGGATGGCTGC
α-SMAb NM_031004 for: GAGGAGCATCCGACCTTGC
rev: ATTTTCTCCCGGTTGGCC

a the first two primer sets were used in conventional PCR, all others primers were used for quantitative real-time PCR analysis on TaqMan.

bthe quantification of α-SMA was done with the SybrGreen dye.