Table 2.
Antibody | Cat no. | Supplier | Speciesa | Dilution |
---|---|---|---|---|
mCCN3/NOV | AF1976 | R&D Systems | m, r | 1:1000, 1:50 (IHC) |
mCCN3/NOV | BF1976 | R&D Systems | m, r | 1:500 (ELISA) |
CCN2/CTGF | 14939 | Santa Cruz | m, r | 1:1000 |
rCollagen type I | PS065 | Monosan, Beutelsbach, Germany | m, r | 1:1000 |
Fibronectin | AB1954 | Millipore, Schwalbach, Germany | m, r | 1:2000 |
α-SMA | CBL1 | Cymbus Biotechn., Hampshire, UK | h, m, r | 1:2000 |
β-Actin | A5441 | Sigma, Taufkirchen, Germany | h, m, r | 1:10000 |
MMP9 | 2270 | Cell Signaling Technology, Frankfurt, Germany | h, m, r | 1:1000 |
TIMP-1 | AF580 | R&D Systems | r | 1:1000 |
Vimentin | ab92547 | Abcam | h, m, r | 1:2500 |
Ribosomal rS6 | 2317 | Cell Signaling Technology | h, m, r | 1:1000 |
ED2 (CD163) | MCA342GA | AbD Serotec, Düsseldorf, Germany | r | 1:50 (IHC) |
phosphoTyr clone 4 G10 | 05-312 | Millipore | h, m, r | 1:1000 |
PDGFR-α | 3164 | Cell Signalling Technology | h, m, r | 1:1000 |
PDGFR-β | 3169 | Cell Signalling Technology | h, m, r | 1:1000 |
Phospho AKT | 4051 | Cell Signalling Technology | h, m, r | 1:1000 |
Total AKT | 2920 | Cell Signalling Technology | h, m, r | 1:1000 |
Phospho ERK1/2 | 9101 | Cell Signalling Technology | h, m, r | 1:1000 |
Total ERK1/2 | 9102 | Cell Signalling Technology | h, m, r | 1:1000 |
Phospho p38 | 612288 | BD Biosciences, Heidelberg, Germany | h, m, r | 1:2500 |
Total p38 | 612168 | BD Biosciences | h, m, r | 1:2500 |
aabbreviations used are: m = mouse, r = rat, h = human