Fig. 3.
A) Representative Western blot for PAR-2 expression in isolated adult cardiac fibroblasts treated for 24 h with either; control, tryptase (100 mU), or tryptase + FSLLRY (PAR-2 receptor antagonist; 10−6 M) media. B) Representative Western blot for COX-2 expression in isolated adult cardiac fibroblasts treated for 24 h with either; control, tryptase (100 mU), or tryptase + FSLLRY (PAR-2 receptor antagonist; 10−6 M) media. C) Densitometric analysis of PAR-2 and COX-2 expression in control, tryptase, tryptase + FSLLRY treated isolated cardiac fibroblasts. Values normalized to GAPDH expression and expressed as mean ± SEM. * denotes p ≤ 0.05 vs. control