Fig. 4.

Stonin 2 interacts with AP-2 through WVxF motifs. (A) Putative AP-2-binding motifs found in stonins and other endocytotic proteins. (B) Stonin-derived peptides interact with AP-2. Material affinity-purified from rat brain by using GST-fused peptides encompassing putative AP-2-binding motifs (see A) was analyzed by immunoblotting for AP-2α, heat shock cognate protein 70, and clathrin (CLC). (C) Transferrin internalization in COS7 cells overexpressing ΔWWW mutant, HA-stonin 2, or its N-terminal domain. (Scale bar, 20 μm.)