FIG. 1.

Replication kinetics of HIV RT variants. PHA-stimulated PBM cells (10×10⁶) were infected with each virus containing 100 TCID₅₀/1×10⁶ PBM cells, and culture supernatants were collected at various time points to measure replication efficiencies of mutant viruses. Both HIV-1 antigen p24 (A) and RT activity (B) were measured at various time points. Replication plot shows that all of the mutants were attenuated in comparison to WT virus, but PSD5.2 replicated efficiently and similarly to WT virus. The results show the mean of three independent assays. Three replicates for each dilution at different time points were used in antigen p24 ELISA, and all RT assays included two replicates for each time point. Color images available online at www.liebertonline.com/vim