. 2012 Feb;25(1):12–20. doi: 10.1089/vim.2011.0057

Table 3.

Fold Change in the Expression of Anti-Apoptosis Marker Genes in PBM Cells Infected with Wild-Type (WT) and Mutant Viruses with Respect to Uninfected Cells

	WT		K65R		M184V		PSD 5.1^a		PSD 5.2^b
Anti-apoptosis marker genes	Day 1	Day 7	Day 1	Day 7	Day 1	Day 7	Day 1	Day 7	Day 1	Day 7
AKT1	1.05	−1.81	−4.82	−1.18	1.78	−1.42	1.89	−1.52	2.02	1.02
BAG1	1.47	−1.32	3.32	1.04	2.91	1.68	2.57	1.49	2.18	−1.27
BAG3	−5.00	−2.13	−6.44	−1.32	−4.10	−1.57	−3.08	−2.47	−3.39	−1.43
BCL2	−2.05	1.01	−2.51	1.65	−2.06	1.02	−2.40	−1.17	−2.50	−1.11
BCL2A1	−1.36	−1.28	−1.16	−1.19	−1.01	−1.40	1.11	−1.14	1.89	1.63
BCL2L1	1.88	2.27	−2.74	2.36	1.97	2.36	2.36	1.69	1.32	1.65
BCL2L2	−2.15	−2.35	−2.48	−1.36	−2.83	−6.98	−1.72	−2.30	−1.73	−4.49
BFAR	−3.58	−4.27	−13.94	−4.74	−3.04	−4.65	−3.23	−4.18	1.70	1.13
NAIP	−2.55	−3.16	−6.66	−3.44	−3.33	−2.32	−2.91	−4.60	−1.53	−3.03
BIRC2	−1.35	−1.50	−11.84	−1.20	−1.28	−1.66	−1.28	1.61	1.26	1.03
BIRC3	−1.54	1.38	−3.95	1.65	−1.29	1.45	−1.27	1.26	−1.13	1.26
BIRC6	1.49	2.02	3.85	1.95	1.40	2.24	1.55	1.70	−1.93	1.03
BIRC8	−1.28	1.62	1.42	1.31	−2.06	1.39	−1.04	1.14	1.15	1.20
BNIP1	−2.95	−2.20	−15.46	−1.38	−1.71	−1.36	−2.03	−2.12	−1.06	1.52
BNIP2	2.11	1.85	1.27	2.25	2.17	1.73	1.96	1.37	1.10	−1.10
BNIP3	−19.56	−2.59	−39.89	−2.14	−14.59	−2.58	−16.68	−3.31	−11.82	−1.49
BRAF	−1.68	−1.27	1.25	3.29	1.57	1.08	1.03	−1.41	1.44	1.09
CASP2	1.43	1.60	−1.98	1.82	1.32	1.65	1.64	−1.12	1.39	−1.04
CFLAR	−3.62	−1.65	-17.86	−1.58	−2.62	−1.81	−2.70	−1.73	−1.62	1.06
FAS	−1.40	1.61	−3.98	1.81	−1.53	1.59	−1.19	1.21	−1.29	1.66
IGF1R	−1.42	1.78	−1.51	2.43	−1.22	1.50	−1.33	1.17	−1.67	−1.15
MCL1	1.38	1.78	3.90	2.04	1.51	1.74	1.70	1.38	1.05	1.09
TNF	2.04	1.32	4.89	1.25	2.08	−1.00	2.72	−1.47	1.92	1.48
CD27	−1.42	−1.03	1.84	1.29	−1.26	1.03	1.19	−1.03	−1.21	−1.18
No. of genes up-regulated/no. of genes downregulated	8/16	11/13	8/16	14/10	9/15	13/11	11/13	9/15	12/12	14/10

^{^a}

PSD 5.1 (pNL4-3) contains RT fragment from a clinical isolate containing K65R, D67N, S68G, K70R, K103N, T215Y, and K219E mutations.

^{^b}

PSD5.2 is a variant of PSD5.1 and lacks the K65R mutation. This variant replicates efficiently in PBM cells (see Fig. 1).

Numbers in bold indicate > 1.5-fold increased expression of specific AAMG on day 7 post-infection of PBM cells by mutant viruses in comparison to WT virus.

Minus sign before numbers indicates downregulation.

PBM, peripheral blood mononuclear; AAMG, anti-apoptosis marker gene.