Table 4.

Fold Change in the Expression of Anti-Apoptosis Marker Genes in Relation to Wild-Type (WT) Virus

	K65R		M184V		PSD 5.1a		PSD 5.2b
Anti-apoptosis marker genes	Day 1	Day 7	Day 1	Day 7	Day 1	Day 7	Day 1	Day 7
AKT1	−5.06	1.53	1.69	1.28	1.80	1.19	1.93	1.84
BAG1	2.26	1.37	1.99	2.22	1.75	1.97	1.49	1.04
BAG3	−1.29	1.61	1.22	1.35	1.62	−1.16	1.47	1.48
BCL2	−1.22	1.63	−1.01	1.01	−1.17	−1.18	−1.22	−1.12
BCL2A1	1.18	1.08	1.35	−1.09	1.51	1.13	2.57	2.10
BCL2L1	−5.15	1.04	1.05	1.04	1.26	−1.34	−1.42	−1.37
BCL2L2	−1.16	1.72	−1.32	−2.97	1.25	1.02	1.24	−1.91
BFAR	−3.89	−1.11	1.18	−1.09	1.11	1.02	6.10	4.83
NAIP	−2.61	−1.09	−1.30	1.36	−1.14	−1.46	1.66	1.04
BIRC2	−8.76	1.25	1.06	−1.11	1.05	−1.07	1.71	1.54
BIRC3	−2.56	1.19	1.19	1.05	1.22	−1.09	1.37	−1.10
BIRC6	2.59	−1.03	−1.06	1.11	1.04	1.19	−2.87	−1.97
BIRC8	1.82	−1.24	−1.61	−1.16	1.23	−1.43	1.48	−1.35
BNIP1	−5.24	1.59	1.73	1.61	1.46	1.04	2.79	3.34
BNIP2	−1.67	1.22	1.03	−1.07	−1.08	−1.35	−1.93	−2.04
BNIP3	−2.04	1.21	1.34	1.01	1.17	−1.28	1.66	1.74
BRAF	2.10	4.16	2.65	1.37	1.74	−1.11	2.42	1.38
CASP2	−2.82	1.14	−1.08	1.03	1.15	−1.78	−1.03	−1.67
CFLAR	−4.93	1.04	1.38	−1.10	1.34	−1.05	2.23	1.75
FAS	−2.85	1.12	−1.10	−1.01	1.18	−1.33	1.08	1.03
IGF1R	−1.06	1.36	1.17	−1.19	1.07	−1.52	−1.17	−2.05
MCL1	2.83	1.14	1.10	−1.03	1.24	−1.29	−1.31	−1.64
TNF	2.40	−1.05	1.02	−1.32	1.34	−1.94	−1.06	1.12
CD27	2.62	1.33	1.13	1.06	1.69	−1.00	1.17	−1.15
No. of genes upregulated >1.5-fold with respect to WT virus	6		2		1		8

^aPSD 5.1 (pNL4-3) contains RT fragment from a clinical isolate containing K65R, D67N, S68G, K70R, K103N, T215Y, and K219E mutations.

^bPSD5.2 is a variant of PSD5.1 and lacks the K65R mutation. This variant replicates efficiently in PBM cells (see Fig. 1).

Numbers in bold indicate > 1.5-fold increased expression of specific anti-apoptosis marker genes observed both on day 1 as well as day 7 by mutant viruses in comparison to WT virus (transient up-regulation on day 1 only is not highlighted in bold).

Minus sign before numbers indicates downregulation.