Table 3.
Tmholo and TmPLP values of AGT-Ma, AGT-Mi and pathogenic variants.
| Enzymatic species | Tmholoa(°C) CD222 nm |
TmPLPa(°C) CD420 nm |
Tmholoa(°C) DSF |
|---|---|---|---|
| AGT-Ma | 77.5 | 78 | 78.6 |
| AGT-Mi | 73.6 | 73.6 | 74.1 |
| W108R-Mi | 54.1 | 53.4 | 53.9 |
| S158L-Ma | 67 | 64 | 64.7 |
| G161S-Mi | 62.5 | 62.8 | 61.8 |
| G161C-Mi | 64 | 64.2 | 63.8 |
| D183N-Ma | 80.8 | 79.6 | 80.1 |
| S187F-Ma | 76.7 | 74.4 | 73.6 |
| S218L-Ma | 57.8 | 57.9 | 56 |
| P319L-Ma | 77.9 | 76.3 | 77.5 |
| G350D-Mi | 73.5 | 73.7 | 72.6 |
a
They represent apparent Tm values and are the mean of two independent experiments. The error is within ± 0.3 °C.