Table 4.
Tmapo values of AGT-Ma, AGT-Mi and pathogenic variants.
| Enzymatic species | Tm1apoa(°C) CD 222 nm |
Tm2apoa (°C) CD 222 nm |
Tm1apoa (°C) DSF |
Tm2apoa (°C) DSF |
|---|---|---|---|---|
| AGT-Ma | 61.1 | 66.4 | 61.9 | 67.3 |
| AGT-Mi | 53.1 | 66 | 54.2 | 66.5 |
| W108R-Mi | 48.9 | 54 | 53.3 | |
| S158L-Ma | 65.9 | 64.3 | ||
| G161S-Mi | 46.8 | 46.5 | ||
| G161C-Mi | 51.1 | 51.3 | ||
| D183N-Ma | 70.2 | 70.3 | ||
| S187F-Ma | 48.2 | 45.3 | ||
| S218L-Ma | 50.9 | 57.8 | 47.2 | 55.7 |
| P319L-Ma | 61.6 | 60.1 | ||
| G350D-Mi | 53.6 | 52.5 |
a
They represent Tm values and are the mean of two independent experiments. The error is within ± 0.3 °C.