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Stimulation of mouse ES cells with exogenous ATP leads to necrosis. Release of lactate dehydrogenase (LDH) as a function of total cellular LDH (plotted as % total cellular LDH) for mouse ES cells transfected with 20 nM non-targeting siRNA (NT siRNA) or P2X7-targeted siRNA (P2X7 siRNA) as indicated (n = 3). Cells stimulated with ATP for 45 min at 37 °C. * P < 0.05 by unpaired two-tailed t test.
