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. 2004 Jan 14;101(4):1004–1009. doi: 10.1073/pnas.0307858100

Fig. 1.

Fig. 1.

Antigen-regulated relocalization of Bcl10 to the T cell/APC interface. (a) OTII T cells were incubated with CHb B cells (APC) for 45 min in the absence (CHb + No antigen) or presence of 10 μg/ml OVA 323–339 (CHb + Ovalbumin). Images of T cell/APC conjugates are arranged as follows (left to right): Nomarski, anti-Bcl10 (red), anti-PKCθ (green), anti-CD4 (blue), and an overlay of the three fluorescent images. (b) Quantitative representation of anti-Bcl10 staining data from a. Images are represented by using a pseudocolor intensity scale (red, most intense staining; dark blue, least intense; scale bar indicates range). Images have been scaled such that the maximal intensity is set as the highest value of anti-Bcl10 staining in the T cell (i.e., APC staining was not considered). (c) Quantification of maximal T cell Bcl10 fluorescence intensity data, with error bars representing SEM. Note that the 7-fold increase in maximal Bcl10 enrichment of ovalbumin-stimulated cells relative to unstimulated cells underestimates the average increase, because two of eight ovalbumin-stimulated cells displayed Bcl10 fluorescence above the maximal detection limit (65,534).