Table 1.
Composition of internal solutions* (in mM)
HEK cells | DRG neurons | Chromaffin cells | |
---|---|---|---|
NaCl | 10 | — | — |
KCl | 145 | 150† | — |
CsCl | — | — | 153 |
MgCl2 | 1 | 1 | 1 |
Hepes | 5 | 10 | 5 |
ATP-Mg | — | 4 | — |
pH | 7.2 | 7.2 | 7.2 |
For fluorescence calibration experiments, 1 mM fura-2 potassium salt was added to internal solutions. For calcium imaging experiments, 0.1 mM fura-2 salt was added to internal solutions.
For capacitance measurement of DRG neurons, 150 mM KCl was replaced with 153 mM CsCl to block K+ channels.