Fig. 1.
Expression of PrP-mLRP2 transgene. (A) Expression of mLRP2 was highest in the brain, and, within the brain, mLRP2 was expressed in multiple regions. (B) mLRP2 staining pattern (Right) was similar to that of endogenous LRP (Left). (C) The overall LRP expression in the brain of mLRP2 TG mice was 3.7-fold higher than in wild-type mice as detected by an antibody to the tail region of LRP, which is identical between endogenous LRP and mLRP2. (D) mLRP2 was effectively processed to the cell surface as shown by cell-surface biotinylation. (E) mLRP2 overexpression increased receptor-mediated 125I-RAP internalization by primary neuronal cultures.