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. 2004 Jan 19;101(4):1087–1092. doi: 10.1073/pnas.0304827101

Fig. 4.

Fig. 4.

Effects of catalytic subunit PKC and Gβγ on WT and mutant GIRK channels. Single-channel currents were studied in inside-out patches in the same condition as Fig. 2. (A) Exposure of the internal patch membrane to the catalytic subunit of PKC (0.1 unit/ml for 20 s) strongly inhibited the WT channels (*, P < 0.05 in comparison with the control group WT without PKC) whereas PKC did not significantly reduce activity of the GIRK1S185A/GIRK4S191A mutant in comparison with that in the absence of PKC (P > 0.05, n = 4–10). NPo.CTL = baseline control NPo. (B) A 20-s exposure of the internal patch membranes to 20 nM Gβγ markedly enhanced channel activity of the GIRK1S185A/GIRK4S191A mutant in comparison with the WT GIRK1-GIRK4 in the absence of G protein in A. No statistical difference was found between WT and mutant groups although the mutant seems to run down faster (n = 5–6).