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. 2004 Jan 19;101(4):1087–1092. doi: 10.1073/pnas.0304827101

Fig. 5.

Fig. 5.

Mediation of substance P-induced inhibition of GIRK channels by PKC. (A) The GIRK1/GIRK4 channel coexpressed with NK1 receptor was inhibited by 1 μM SP. After the inhibition, 30 nM PMA had no further inhibitory effect. (B) Mutation of Ser-185 in the GIRK4 and Ser-191 in the GIRK4 to alanine eliminated the SP- and PMA-induced channel inhibition. (C) SP inhibited the heteromeric GIRK1/GIRK4 channel coexpressed with NK1 receptor. The inhibition was not seen in the mutants with alanine or aspartate at these putative phosphorylation sites, in the presence of specific PKC blockers, or in the GIRK1/GIRK4 expressed without the NK1 (n = 4–12).