Fig. 1.
Generation of transgenic hy2 lines expressing PCB:ferredoxin oxidoreductase (PcyA). (A) HY2 and cyanobacterial PcyA are ferredoxin-dependent bilin reductases that respectively convert BV IXα to PϕB and PCB. (B) Construct used for PcyA expression in hy2 mutant plants. 35S, cauliflower mosaic viral 35S promoter; NPTII, kanamycin resistance gene; TP, transit peptide from soybean rbcS; pAnos, terminator from nopaline synthase. (C) Ten-day-old seedlings of Ler WT, hy2, and three T3 PcyA-expressing hy2 lines grown under long-day (LD) conditions (16 h of white light at 150 μmol·m-2·s-1 and 8 h of darkness) are shown. (D) RNA blot analysis of total RNA (10 μg) isolated from Ler WT and PCYA transgenic lines by using a Synechocystis pcyA transcript as a probe. The RNA blot was reprobed with an ACT8 transcript to show equal loadings (53).