Fig. 2.

Comparative spectroscopic and biochemical properties of phyA-containing extracts from Ler WT, hy2, phyA, phyB, and PCYA1 seedlings. (A) Phytochrome difference spectra of 7-day-old dark-grown Ler WT (dotted line), PCYA1 (solid line), and hy2-106 (dotted–dashed line) seedling extracts. (B) Zinc blot (Left) and phyA immunoblot (Right) analysis of partially purified protein extracts (2 μg per lane) from 5-day-old etiolated seedlings that had been treated for 24 h with darkness (D), white fluorescence light (W; 150μmol·m^-2·s^-1), R-LED light (657 nm; 20 μmol·m^-2·s^-1), or FR-LED light (734 nm; 10 μmol·m^-2·s^-1) before extraction. See Materials and Methods for details.