Figure 4.
In vivo cell transplantation studies with reprogrammed DA neuron-like cells. (A) About 3 × 105 reprogrammed cells (12 days post infection) were injected into the middle of striatum of rats to the side with 6-OHDA-induced lesions with a Hamilton syringe. Right before and at different times after cell transplant, rats were evaluated for amphetamine-induced rotational behavior with a rotometer. Error bars represent SEM, n = 8. (B) Rotational behaviors of four 6-OHDA-lesioned rats injected with hiDA cells. (C) Brain tissues from the two rats that showed consistent correction of amphetamine-induced rotational behaviors were examined for evidence of transplanted cells through immunoflurescenece. HN: staining for human nuclear protein, which is an indicator of injected human cells. Scale bars represent 200 μm.