Table 2.

Effect of tamoxifen (TM) treatment on tissue weights, testicular transcript levels and serum hormone levels in AR^flox/y control animals

	dosage TM / day	1 mg	3 mg	3 mg	3 mg
sacrifice on day	day 55	day 55	day 61	day 104
tissue weights	kidney	105.2 ± 4.9	99.2 ± 3.1	94.9 ± 4.8	117.1 ± 13.1
	testis	104.9 ± 3.3	95.7 ± 2.9	102.8 ± 1.6	94.1 ± 6.6
	epididymis	104.9 ± 4.5	90.8 ± 4.5	83.2 ± 5.8*	95.3 ± 4.0
	seminal vesicle	108.0 ± 8.0	118.6 ± 5.9	82.6 ± 12.2	89.6 ± 3.4
	anterior prostate	109.4 ± 7.7	105.2 ± 7.6	105.9 ± 16.6	73.9 ± 7.8
	ventral prostate	95.2 ± 10.7	114.0 ± 12.7	104.3 ± 7.3	60.6 ± 5.0*
q-RT-PCR on testis extracts	AR	82.6 ± 8.5	112.3 ± 7.8	82.4 ± 15.2	111.4 ± 21.8
	Rhox5	103.6 ± 11.6	109.0 ± 6.1	108.2 ± 20.1	111.5 ± 9.4
	Spinlw1	137.4 ± 18.2	115.9 ± 6.1*	91.3 ± 11.0	138.0 ± 11.0*
	StAR	14.1 ± 1.7*	26.7 ± 3.7*	7.5 ± 0.9*	82.9 ± 8.7
	Cyp17a1	59.0 ± 8.7*	70.9 ± 4.9*	59.4 ± 4.1*	99.2 ± 10.0
	Insl3	65.8 ± 8.8	58.9 ± 5.3*	11.9 ± 2.0*	29.4 ± 5.9*
serum level	FSH	ND	76.7 ± 4.1*	ND	96.7 ± 15.0
	LH	ND	35.4 ± 3.4*	ND	242.6 ± 74.8
	Basal testosterone	ND	11.3 ± 6.6*	ND	25.4 ± 10.4
tubular diameter		ND	104.3 ± 2.0	101.3 ± 3.9	95.4 ± 1.2*

AR^flox/y control mice were treated with vehicle (V) or tamoxifen (TM; 1 or 3 mg/day) from day 50 on for five consecutive days. Tissue weights, testicular transcript levels (measured by qPCR) and serum hormone levels were measured one day after treatment (day 55), seven days after treatment (day 61) and 50 days after treatment (day 104). The data shown (mean ± SEM) represent values in AR^flox/y mice treated with TM expressed relative to values observed in AR^flox/y mice treated with V (arbitrarily assigned a value of 100). Statistical analysis was performed by ANOVA supplemented with a Fisher LSD test. Values that differ significantly (p ≤ 0.05) are indicated by an asterisk. ND = not determined.