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. 2011 Oct 1;1(4):354–365. doi: 10.4161/spmg.1.4.18902

Figure 1.

Figure 1

TAP 14-3-3 transgene construct plan and TAP 14-3-3 expression analysis in testis. (A) A Diagrammatic representation of the TAP 14-3-3 cDNA construct. (B) The TAP tag consisted of a protein A tag and a calmodulin binding peptide (CBP) separated by the TEV cleavage site sequence. The TAP tag was fused to 14-3-3ζ, cDNA at its N-terminal end. (C) Protein gel blot analysis of 14-3-3 expression in testis. Both the wild type and transgenic mice testes extracts showed endogenous 14-3-3 protein migrating at 30 kDa whereas TAP 14-3-3 (50 kDa) could be detected only in the testis extracts of transgenic mice. (D) Immunohistochemical analysis of the TAP 14-3-3 transgenic mice testes showed that the TAP 14-3-3 is expressed in both the seminiferous tubules and the interstitial compartment of the testis. Testis sections from the wild type mice stained with rabbit IgG showed no fluorescence.