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. 2011 Oct 1;1(4):354–365. doi: 10.4161/spmg.1.4.18902

Figure 5.

Figure 5

SPATA 18 interacts with 14-3-3 and B-raf. (A) HEK293 cells were transfected with pcDNA3.1 His-SPATA18 and pcDNA5.1 Myc-14-3-3 as described in materials and methods. The cell extracts were prepared 48 h after transfection and were subjected to Immunoprecipitation with the His-tag antibody and a control antibody. Immunoblotting of His-SPATA18 immuoprecipitates showed that Myc-14-3-3 (31 kDa) co-precipitate with His-SPATA18 (∼75 kDa). (B) HEK-293 cells were transfected with pcDNA3.1His-SPATA18 plasmid. The His-SPATA18 protein was immunoprecipitated with His-tag antibody and the immunoprecipation eluate was analyzed with anti-His and B-raf antibodies. B-raf (∼85 kDa) co-precipitated with His-SPATA18.