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Conjugation of adjudin to KLH (keyhole limpet hemocyanin, an adjuvant), and its use as an immunogen for anti-adjudin antibody production. KLH was first reacted with SFB (succinimidyl 4-formylbenzoate, a heterobifunctional crosslinker) to generate a benzaldehyde at its N-terminus. This modified KLH was then reacted spontaneously in physiological buffer with the hydrazide group in adjudin, forming a stable KLH-adjudin conjugate via a hydrazone linkage.
