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Identification of actin and myosin as molecular targets of adjudin in the rat testis by co-immunoprecipitation and mass spectrometry. (A) Actin and myosin were found to bind adjudin as determined by co-immunoprecipitation using affinity chromatography with an anti-adjudin IgG-Protein A resin, coupled with SDS-PAGE, Coomassie blue gel staining and mass spectrometry. M, molecular weight marker. (B) Sequences corresponding to actin (i) and myosin (ii) as obtained by mass spectrometry.
