Figure 6.

Characterization of actin as a molecular target of adjudin. (A) Adjudin binds to actin (42 kDa) as determined by co-immunoprecipitation. Aliquots (lane 1, 10 µl; lane 2, 30 µl) of the eluant (Fig. 5A) were resolved by SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting using anti-actin IgG. Ctrl, testis lysate (20 µg). (B) The interaction of adjudin with actin was specific since unlabeled (i.e., cold) adjudin displaced the binding of [³H]-adjudin to actin IgG in a competitive binding assay. Error bars represent mean ± SD from three different experiments. *p < 0.05; ns, not significant. (Student's t-test).