Figure 7.

Characterization of testin as a molecular target of adjudin. (A) Adjudin binds to testin (35 and 37 kDa) as determined by co-immunoprecipitation. Aliquots (lane 1, 10 µl; lane 2, 30 µl) of the eluant (Fig. 5A) were resolved by SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting using a monospecific anti-testin antibody.²²,²³ Ctrl, testis lysate (100 µg). (B) The interaction of adjudin with testin was specific since unlabeled (i.e., cold) adjudin displaced the binding of [³H]-adjudin to testin IgG in a competitive binding assay. Error bars represent mean ± SD from three different experiments. *p < 0.05. (Student's t-test).