Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of W-7 and Casodex on AR binding to CaM on CaM-agarose affinity column. Whole-cell lysates from exponentially growing LNCaP cells were treated with 20 μM W-7 (▴), 100 μM Casodex (▪), or control (•) before applying to a CaM-agarose affinity column as described in Materials and Methods. (A) Elution profile of proteins. (B) Western blot analysis of AR and cyclin A in equal volumes of indicated fractions. Preload, cell extract; FT, flow-through; W, wash; E, eluate.
