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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Cancer Res. 2011 Feb 18;71(6):2087–2097. doi: 10.1158/0008-5472.CAN-10-1511

Figure 3. PKCδ is not required for activation of Ras or PDK1/AKT in NSCLC cells.

Figure 3

(A) Ras activation in control (scr), δ193, and δ203 expressing A549, H2009 and H441 cells. Top, pull-down of GTP-bound Ras; far left, GTPγS or GDP was added to cell lysates prior to pull-down. Second, third and fourth rows show immunoblots for total Ras, PKCδ and actin. (B) Cell lysates prepared from control (scr), δ193, and δ203 expressing K-Ras independent (A549 and H460) and K-Ras dependent (H2009 and H441) NSCLC cells were immunoblotted for phospho (S241) PDK-1, phospho (S473) AKT, phospho (S217/221) as indicated. Blots were stripped and probed for total PDK-1, AKT, PKCδ and actin, as indicated. Experiments were repeated a minimum of 4 times.