(A) Ras activation in control (scr), δ193, and δ203 expressing A549, H2009 and H441 cells. Top, pull-down of GTP-bound Ras; far left, GTPγS or GDP was added to cell lysates prior to pull-down. Second, third and fourth rows show immunoblots for total Ras, PKCδ and actin. (B) Cell lysates prepared from control (scr), δ193, and δ203 expressing K-Ras independent (A549 and H460) and K-Ras dependent (H2009 and H441) NSCLC cells were immunoblotted for phospho (S241) PDK-1, phospho (S473) AKT, phospho (S217/221) as indicated. Blots were stripped and probed for total PDK-1, AKT, PKCδ and actin, as indicated. Experiments were repeated a minimum of 4 times.