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. 2011 Dec 7;158(2):1018–1033. doi: 10.1104/pp.111.184572

Figure 2.

Figure 2.

PpPDK1 rescues lethality caused by the deletion of both S. cerevisiae PDK1 homologs, PKH1 and PKH2. Yeast strain AC306 was transformed with p416GPD containing the indicated PDK1 cDNAs. NT, Untransformed AC306; EV, empty vector-transformed AC306. Sporulation was induced, and the four haploid cells produced by each diploid cell were grown on rich medium (YPD) and medium containing 5-FOA. Spore phenotypes are indicated on the left and were determined by replica plating on medium lacking His, Trp, or uracil. A, On YPD plates, pkh1Δ::HIS3/pkh2Δ::TRP1 spores are only viable when AtPDK1, SlPDK1, or PpPDK1 is expressed. B, α-MBP western blot showing protein expression. C, On 5-FOA plates, pkh1Δ::HIS3/pkh2Δ::TRP1 spores are not viable due to loss of the URA3-marked p416GPD plasmid. wt, Wild type.