Figure 2.

Transcriptional regulation of TvPirin. RNA was extracted from T. versicolor seedlings after exposure to water, DMBQ, or M. truncatula (host) roots for 2 h. Steady-state transcript levels of TvPirin were determined by qRT-PCR and normalized to the constitutively expressed gene TvQN8 for each sample. Data are means ± sd of three technical replicates of three biological replicates (n = 9). Each biological replicate was a square petri dish with 20 to 50 seedlings. Expression levels of roots treated with DMBQ was set to 100%. Note the relative expression values are written on top of each bar.