Table I.
Efficiency and phenotypes of transgenic rootsa
Phenotype | pHG8-YFP | pHpPRN | t-Value |
Transformation efficiencyb | 28 ± 2.2 | 26 ± 2.8 | 0.61 |
n = 1271 | n = 1539 | ||
YFP-positive roots per plantc | 1.8 ± 0.4 | 1.7 ± 0.4 | 0.88 |
n = 352 | n = 403 | ||
YFP-positive roots per plantd | 2.0 ± 0.9 | 2.4 ± 0.7 | 0.05 |
n = 49 | n = 57 | ||
Root length (cm)d | 3.5 ± 1.8 | 3.1 ± 1.0 | 0.74 |
n = 49 | n = 57 | ||
Lateral roots per YFP rootd | 0.1 ± 0.3 | 0.1 ± 0.4 | 0.20 |
n = 49 | n = 57 | ||
Root growth no DMBQ (mm/24 h)d | 2.6 ± 0.4 | 2.5 ± 0.6 | 0.85 |
n = 40 | n = 40 | ||
Root growth with DMBQ (mm/24 h)d | 2.7 ± 0.5 | 2.6 ± 0.7 | 0.12 |
n = 40 | n = 40 |
Values are mean ± sd with n = total number of plants or roots in all replicates. Means are the averages of three independent transformation experiments per construction. Data were analyzed using SAS statistical software (pooled t test/equal/unequal variance).
Percentage of plants with at least one yellow root.
Twenty-one days after transformation.
Thirty-three days after transformation.