Table II.
Haustorium development in transgenic roots silenced for TvPIRIN
Treatmenta | Percentage of Roots with Haustoria | ||
Seedlingsb | pHG8YFP | pHpPRN | |
30 μM DMBQ | 75 ± 11 | 71 ± 12* | 38 ± 10** |
n = 565 | n = 532 | n = 505 | |
Host root exudates | 85 ± 3 | 73 ± 9* | 18 ± 5** |
n = 214 | n = 66 | n = 27 | |
Host roots | 87 ± 8 | 84 ± 9* | 47 ± 10** |
n = 90 | n = 113 | n = 23 | |
Water | 0 ± 0 | 0 ± 0 | 0 ± 0 |
n = 186 | n = 104 | n = 97 |
T. versicolor roots were exposed to DMBQ or host exudates and assayed 24 h later or exposed to Arabidopsis roots and assayed after 5 d. Data are averages ± sd of three to seven plates with four to six independently transformed plants in each plate. Experiment with DMBQ treatment was repeated three times with plants from three independent transformation experiments. Each plant had 1 to 30 roots depending on the age of the plant (n = total number of roots in all replicates). Each treatment was applied to similarly aged plants from the same transformation experiment. Pairwise comparisons of treatments within a row labeled with a different number of asterisks are significantly different at α = 0.05 (t test).
Seedling data were not included in statistical analysis because they were differently aged.