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. 2011 Dec 8;158(2):1067–1078. doi: 10.1104/pp.111.188532

Figure 4.

Figure 4.

NO3− influx, NRT2.1, and NAR2.1 mRNA level in roots of wild-type and transgenic plants in response to darkness. A to C, Plants were grown in vitro for 8 d on 0.2 mm KNO3 as N source and harvested after 14 h of light (white bars) or 10 h of light + 4 h of darkness (dark bars). D to F, Plants were grown in vitro for 7 d on 0.2 mm KNO3 as N source and transferred, after 8 h of light, either in continuous dark (dark bars) or in continuous light (white bars) for 24 h. A and D, Root NRT2.1 expression quantified by Q-PCR. Values are means of two biological replicates ± sd. B and E, Root NO3− influx measured at the external concentration of 0.2 mm 15NO3−. Values are means of 12 replicates ± sd. C and F, Root NAR2.1 expression quantified by Q-PCR. Values are means of two biological replicates ± sd. Differences between plants treated in the light and in the dark are significant at *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test).