Fig. 2.

Time course of gene expression by RT-PCR for the fdx and fpr complement of S. coelicolor A3(2). Total RNA was extracted and used as a template with each pair of specific primers 3, 6, 12, and 24 h postgermination. Products were authenticated by sequencing. In negative controls, containing DNA polymerase but lacking reverse transcriptase, amplified products were not detected.