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GPC separation of ISA complexes. ISA1 was partially purified by AEC from 20-DAP endosperm of the indicated genotype and then fractionated further by GPC. ISA1 in the eluate fractions was detected by immunoblot analysis. An equal volume of each fraction was loaded on the SDS-PAGE gel used for the immunoblot. The elution volumes of standards of known molecular mass are indicated.
